ISO INTERNATIONAL STANDARD 10504 Second edition 2013-09-01 Starch derivatives Determination of the composition of glucose syrups, fructose syrups and hydrogenated glucose syrups Method using high performance liquid chromatography Produits dérives de I'amidon -Détermination de la composition des sirops de glucose, des sirops de fructose et des sirops de glucose hydrogénés - Méthode par chromatographie en phase liquide a haute performance Reference number IS0 10504:2013(E) ISO IS02013 I without license from IHS Not for Resale IS0 10504:2013(E) COPYRIGHTPROTECTEDDOCUMENT @ISO2013 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56:CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ IS0 2013 - All rights reserved No reproductic networking permited without license from IHS Not for Resale IS0 10504:2013(E) Contents Page Foreword ..iv 1 Scope .1 2 Normative references 3 Principle. 4 Reagents 5 2 Apparatus 6 Procedure .3 6.1 Choice of column .3 6.2 System start-up. .3 6.3 Calibration of column. .3 6.4 Sample preparation 6.5 Sample analysis .4 7 Calculation .4 8 Precision .5 8.1 Repeatability 8.2 Reproducibility .5 Annex A (informative) Examples of standard solutions. 7 iii ded by IHS under license with ISt ed without license from IHS Not for Resale