论文标题
用倾斜的后刷毛细管镜在体内成像人体血细胞
Imaging human blood cells in vivo with oblique back-illumination capillaroscopy
论文作者
论文摘要
我们提出了一种非侵入性的，无标签的方法，它使用倾斜的后刷毛细血管镜（OBC）在体内通过人毛细血管流动的血细胞进行成像。绿光照明允许同时进行相位和吸收对比度，从而增强了区分红色和白细胞的能力。通过目标镜头通过物镜的单面照明可以通过紧密的照明检测分离和简化的设置实现200 Hz成像。当照明轴从检测轴抵消大约225 um时，将相对造影剂优化，当对80 um和人体腹舌深度成像时。我们展示了单个红细胞的高速成像，白细胞细胞细节的白细胞以及流经人舌的毛细血管和血管的血小板。放置在物镜上的自定义气帽稳定了视场，从而使单个毛细管的纵向成像长达七分钟。我们呈现Fitzpatrick皮肤光谱II，IV和VI的个体中血细胞的高质量图像，表明该技术对高外周黑色素浓度具有鲁棒性。这种方法的信号质量，速度，简单性和鲁棒性强调了其非侵入性血细胞计数的潜力。
We present a non-invasive, label-free method of imaging blood cells flowing through human capillaries in vivo using oblique back-illumination capillaroscopy (OBC). Green light illumination allows simultaneous phase and absorption contrast, enhancing the ability to distinguish red and white blood cells. Single-sided illumination through the objective lens enables 200 Hz imaging with close illumination-detection separation and a simplified setup. Phase contrast is optimized when the illumination axis is offset from the detection axis by approximately 225 um when imaging 80 um deep in phantoms and human ventral tongue. We demonstrate high-speed imaging of individual red blood cells, white blood cells with sub-cellular detail, and platelets flowing through capillaries and vessels in human tongue. A custom pneumatic cap placed over the objective lens stabilizes the field of view, enabling longitudinal imaging of a single capillary for up to seven minutes. We present high-quality images of blood cells in individuals with Fitzpatrick skin phototypes II, IV, and VI, showing that the technique is robust to high peripheral melanin concentration. The signal quality, speed, simplicity, and robustness of this approach underscores its potential for non-invasive blood cell counting.
