论文标题
细胞对细胞变化的非遗传遗传约束
Non-genetic inheritance restraint of cell-to-cell variation
论文作者
论文摘要
由于细胞内生化过程的随机性以及分裂过程中资源的分布，由于细胞，周期时间，生长速率，蛋白质浓度的物理和功能特征的异质性（例如大小，周期时间，生长速率，蛋白质浓度）在ISEMENIC人群中增殖。相反，它部分受到连续世代之间细胞成分的遗传的限制。在这里，我们介绍了一种新的实验方法，用于测量细菌细胞特征中异质性的增殖，基于测量两个姐妹细胞如何随着时间而彼此不同。我们的测量提供了不同细胞特性的遗传动力学，以及细胞的“惯性”，以维持这些特性。我们发现遗传动力学是特定于属性的，并且可以表现出长期记忆（约10代），可限制细胞之间的变化。我们的结果可以揭示细菌中非遗传遗传的机制，并有助于了解细胞如何控制其性质细胞种群中其特性和异质性。
Heterogeneity in physical and functional characteristics of cells (e.g. size, cycle time, growth rate, protein concentration) proliferates within an isogenic population due to stochasticity in intracellular biochemical processes and in the distribution of resources during divisions. Conversely, it is limited in part by the inheritance of cellular components between consecutive generations. Here we introduce a new experimental method for measuring proliferation of heterogeneity in bacterial cell characteristics, based on measuring how two sister cells become different from each other over time. Our measurements provide the inheritance dynamics of different cellular properties, and the "inertia" of cells to maintain these properties along time. We find that inheritance dynamics are property-specific, and can exhibit long-term memory (~10 generations) that works to restrain variation among cells. Our results can reveal mechanisms of non-genetic inheritance in bacteria and help understand how cells control their properties and heterogeneity within isogenic cell populations.
