论文标题
通过在单个细菌中刺激D2O代谢掺入的拉曼散射成像来快速测定抗菌敏感性
Rapid Determination of Antimicrobial Susceptibility by Stimulated Raman Scattering Imaging of D2O Metabolic Incorporation in a Single Bacterium
论文作者
论文摘要
迫切需要快速的抗菌敏感性测试（AST）来治疗正确的抗生素感染并减缓抗生素耐药菌的出现。在这里，我们报告了一个表型平台，该平台通过飞秒刺激氧化氘（D2O）代谢的拉曼散射成像迅速产生AST。培养物在70％D2O培养基中培养后短短10分钟，将D2O的代谢掺入单个细菌中的生物量，这是当前技术中最快的。单细胞代谢灭活浓度（SC-MIC）在从菌落到结果的不到2.5小时内获得。 37组样品的SC-MIC结果，包括8种主要细菌和14种不同的抗生素经常在诊所中遇到，通过通过肉汤微稀释度盲目测量的标准最小抑制浓度来验证。为了临床翻译，证明了1-H抗生素治疗后D2O代谢掺入和SC-MIC测定的SRS成像，D2O和30分钟的D2O和抗生素在尿液或全血中孵育30分钟。
Rapid antimicrobial susceptibility testing (AST) is urgently needed for treating infections with correct antibiotics and slowing down the emergence of antibiotic-resistant bacteria. Here, we report a phenotypic platform that rapidly produces AST results by femtosecond stimulated Raman scattering imaging of deuterium oxide (D2O) metabolism. Metabolic incorporation of D2O into biomass in a single bacterium is probed in as short as 10 minutes after culture in 70% D2O medium, the fastest among current technologies. Single-cell metabolism inactivation concentration (SC-MIC) is obtained in less than 2.5 hours from colony to results. The SC-MIC results of 37 sets of samples, which include 8 major bacterial species and 14 different antibiotics often encountered in clinic, are validated by standard minimal inhibitory concentration blindly measured via broth microdilution. Towards clinical translation, SRS imaging of D2O metabolic incorporation and SC-MIC determination after 1-h antibiotics treatment and 30-minutes mixture of D2O and antibiotics incubation of bacteria in urine or whole blood is demonstrated.
