论文标题
计算的折纸断层扫描
Computed Origami Tomography
论文作者
论文摘要
在本文中,我们提供了易于构建实验设置的组装说明,以便获得层析成像的实践经验。鉴于这一点,本文可以看作是与出色的数学教科书的补充作品,这是与层析成像的基本数学原理有关的本科层面。由于设置使用光进行断层成像,因此所研究的物体需要像折纸图一样透明。如果读者希望在不组装设备的情况下尝试计算层析成像重建,我们将提供几个对象的数据库以及其层析成像测量和公开可用的软件。此外,在冷冻成像中的最新进展实现了单个颗粒(例如病毒)的三维高分辨率可视化。为了体现和经验,单粒子冷冻电子显微镜我们提供了一个高级组件,我们用来生成模拟冷冻记录的数据。我们还讨论了从冷冻微观数据中重建颗粒的一些主要实际困难。
In this paper, we provide assembly instructions for an easy to build experimental setup in order to gain practical experience with tomography. In view of this, this paper can be seen as a complementary work to excellent mathematical textbooks at an undergraduate level concerned with the basic mathematical principles of tomography. Since the setup uses light for tomographic imaging, the investigated objects need to be light transparent, like origami figures. In case the reader wants to experiment with computational tomographic reconstructions without assembling the device, we provide a database of several objects together with their tomographic measurements and a publicly available software. Moreover, recent advances in Cryo-imaging enabled three-dimensional high-resolution visualization of single particles, such as for instance viruses. To exemplify, and experience, single particle Cryo-electron microscopy we provide an advanced assembly, which we use to generate data simulating a Cryo-recording. We also discuss some of the major practical difficulties for reconstructing particles from Cryo-microscopic data.