论文标题
Carminomycin与合成多核苷核苷酸聚（A）和Poly（U）的结合：吸收和极化荧光研究
Binding of Carminomycin to synthetic polyribonucleotides poly(A) and poly(U): absorption and polarized fluorescence study
论文作者
论文摘要
在广泛的磷酸盐至二元（P/d）范围内研究了蒽环类抗生素癌甲霉素（CM）与合成多核苷酸聚（A）和聚（U）的结合，使用吸收和极化的荧光光谱。已经鉴定出了两种与SS-聚核苷酸的CM结合。它们中的第一个在低磷酸盐与染料（P/D）之比中占主导地位是在聚合物表面上的杂环染料的自组装，该聚合物表面由CM糖部分的氨基糖基团的合作静电结合驱动，以带负电荷的多核苷酸磷酸盐基团与色彩循环自堆组合。在高的P/D值下，堆叠式缔合的人与配体与核碱基的单体结合变得普遍。两种情况下估计结合的热力学参数。
Binding of anthracycline antibiotic carminomycin (CM) to synthetic polyribonucleotides poly(A) and poly(U) was studied in solution of low ionic strength in a wide phosphate-to-dye (P/D) range using absorption and polarized fluorescence spectroscopy. Two different modes of CM binding to the ss-polynucleotides have been identified. The first of them dominating at low phosphate-to-dye (P/D) ratios is self-assembly of the heterocyclic dye on the polymer surface driven by to cooperative electrostatic binding of amino group of CM sugar moiety to negatively charged polynucleotide phosphate groups with the chromophores self-stacking. At high P/D values, the stacking-associates disintegrate and monomeric binding of ligand to nucleic bases become prevalent. Thermodynamic parameters of binding were estimated for the both cases.
