论文标题
提高可靠性，透明度和交联质谱的可及性
Towards Increased Reliability, Transparency and Accessibility in Crosslinking Mass Spectrometry
论文作者
论文摘要
在过去的二十年中，通过多个实验室的平行发展，交联的质谱法（交联MS）已大大成熟为一种方法，证明了其在复杂混合物中的蛋白质结构确定，构象分析和绘制蛋白质相互作用的适用性。交联的MS已成为一种广受赞誉和常规应用的工具，尤其是在结构生物学中。因此，社区及时致力于制定方法论和报告标准。这份白皮书建立在一个开放过程的基础上，该过程包括自2015年以来在社区会议上进行的许多事件，并确定了交联MS的各个方面，该方面应作为交联MS标准计划的一部分制定指南。
Crosslinking mass spectrometry (Crosslinking MS) has substantially matured as a method over the last two decades through parallel development in multiple labs, demonstrating its applicability for protein structure determination, conformation analysis and mapping protein interactions in complex mixtures. Crosslinking MS has become a much-appreciated and routinely applied tool especially in structural biology. Therefore, it is timely that the community commits to the development of methodological and reporting standards. This white paper builds on an open process comprising a number of events at community conferences since 2015 and identifies aspects of Crosslinking MS for which guidelines should be developed as part of a Crosslinking MS standards initiative.
