论文标题
远程聚焦显微镜对放大不匹配的敏感性
Sensitivity of Remote Focusing Microscopes to Magnification Mismatch
论文作者
论文摘要
远程聚焦（RF）是一种极大地扩展光学显微镜的无像差轴向扫描范围的技术。为了最大程度地提高RF系统中的衍射有限深度范围，继电器镜头的放大率应该使目标的瞳孔平面相互准确映射。在本文中，我们研究了RF系统对放大不匹配的耐受性，并量化了在不同聚焦深度处存在的残留球形像差量。我们观察到，与理想放大倍数的小偏差会导致剩余的球形畸变项增加，从而导致动态范围减少。对于高数值孔径目标，模拟预测动态范围的50％减小了1％放大率不匹配。该模拟已针对具有理想和非理想重大的实验RF系统进行了验证。实验确认的预测还提供了一种有价值的经验方法，可以根据焦点两侧的球形畸变的迹象来确定系统何时接近理想的相位匹配条件。
Remote focusing (RF) is a technique that greatly extends the aberration-free axial scan range of an optical microscope. To maximise the diffraction limited depth range in an RF system, the magnification of the relay lenses should be such that the pupil planes of the objectives are accurately mapped on to each other. In this paper we study the tolerance of the RF system to magnification mismatch and quantify the amount of residual spherical aberration present at different focusing depths. We observe that small deviations from ideal magnification results in increased amounts of residual spherical aberration terms leading to a reduction in the dynamic range. For high numerical aperture objectives, the simulation predicts a 50% decrease in the dynamic range for 1% magnification mismatch. The simulation has been verified against an experimental RF system with ideal and non-ideal magnifications. Experimentally confirmed predictions also provide a valuable empirical method of determining when a system is close to the ideal phase matching condition, based on the sign of the spherical aberration on either side of focus.
