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The neural network (NN) based singing voice synthesis (SVS) systems require sufficient data to train well and are prone to over-fitting due to data scarcity. However, we often encounter data limitation problem in building SVS systems because of high data acquisition and annotation costs. In this work, we propose a Perceptual Entropy (PE) loss derived from a psycho-acoustic hearing model to regularize the network. With a one-hour open-source singing voice database, we explore the impact of the PE loss on various mainstream sequence-to-sequence models, including the RNN-based, transformer-based, and conformer-based models. Our experiments show that the PE loss can mitigate the over-fitting problem and significantly improve the synthesized singing quality reflected in objective and subjective evaluations.