论文标题

共聚焦ISO 21073:2019共聚焦显微镜的解释:生物成像的荧光共聚焦显微镜的光学数据 - 推荐的质量控制方法

Interpretation of Confocal ISO 21073: 2019 confocal microscopes: Optical data of fluorescence confocal microscopes for biological imaging- Recommended Methodology for Quality Control

论文作者

Nelson, Glyn, Gelman, Laurent, Faklaris, Orestis, Nitschke, Roland, Laude, Alex

论文摘要

如果不正确维护或控制质量,共聚焦成像系统的性能可能不会比通用广阔的广场系统更好。 ISO 21073的发布,“共聚焦显微镜 - 生物成像的荧光共聚焦显微镜的光学数据”,为应对共焦显微镜进行的最小质量控制(QC)设定了标准。在这里,我们描述了执行QC要求以满足ISO 21073的方法的方法,并提出了应执行的其他QC方法,以获取有关显微镜系统的最低信息水平。

The performance of a confocal imaging system may be no better than a general-purpose widefield system if it is not properly maintained or quality controlled. The publication of ISO 21073, 'Confocal microscopes- Optical data of fluorescence confocal microscopes for biological imaging', set a standard for the minimal Quality Control (QC) that should be performed for Confocal Microscopes. Here we describe methodology for performing the QC requirements to satisfy ISO 21073, as well as suggesting other QC methods that should be performed to obtain a minimum level of information about the microscope system.

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