论文标题
细胞微as子：具有单细胞精度的生物制作
Cellular Micromasonry: Biofabrication with Single Cell Precision
论文作者
论文摘要
在许多组织中，细胞类型在单细胞长度尺度上有所不同，从而产生了生理功能基本的详细空间异质性。为了了解组织功能与详细结构之间的这种关系，以及有一天在结构和生理上精确的组织上，我们需要能够组装有生物组织中细节水平的3D细胞结构的能力。在这里，我们介绍了一种3D单元组件的方法，该方法的精度比单细胞量表更详细。通过这种方法，我们创建了许多具有明确定义的空间模式的结构，表明单个单元格的尺度可以改变细胞类型并在组装后显示功能。该技术为在定义的环境中研究细胞行为提供了无数的机会，包括在胚胎发生过程中运行的复杂相互作用。
In many tissues, cell type varies over single-cell length-scales, creating detailed spatial heterogeneities fundamental to physiological function. To gain understanding of this relationship between tissue function and detailed structure, and to one day engineer structurally and physiologically accurate tissues, we need the ability to assemble 3D cellular structures having the level of detail found in living tissue. Here we introduce a method of 3D cell assembly that has a level of precision finer than the single-cell scale. With this method we create numerous structures having well-defined spatial patterns, demonstrating that cell type can be varied over the scale of individual cells and showing function after their assembly. This technique provides innumerable opportunities to study cellular behavior in defined contexts including complex interactions operating during embryogenesis.
