论文标题
NZVI/PVP纳米颗粒的合成用于生物修复应用
Synthesis of nZVI/PVP nanoparticles for bioremediation applications
论文作者
论文摘要
这项研究的目的是合成和研究用于生物修复应用的零价铁（ZVI）纳米颗粒（NPS）。使用与聚（N-乙烯基吡咯烷酮）（PVP）的亚铁盐溶液进行化学还原来制造ZVI-NP，用作稳定剂。进行合成，并没有超声处理。制造ZVI NP并使用扫描电子显微镜（SEM），透射电子显微镜（TEM），X射线粉末衍射（XRD）分析和傅立叶变换红外光谱（FTIR）来表征。实验观察结果表明，根据合成条件和稳定剂的协调，形成了不同形态的NP。合成的NP的胶体溶液用于九种不同的对照微生物的抗菌活性测试和生物膜形成测定：大肠杆菌（ATCC 25922），铜绿假单胞菌（ATCC 15692），Enteroccus faeccus faecalis（ATCC 15692）分离株），proteus ufgaris（实验室分离株），金黄色葡萄球菌（ATCC 29213），蜡状芽孢杆菌（DSMZ 4312），枯草芽孢杆菌（ATCC 6633）和念珠菌（ATCC 10231）。所有对照菌株均未显示出未经超声处理未经超声处理的抗菌作用，均不针对PVP稳定的ZVI NP。然而，生物膜结果表明，在对照孔中测试了微生物的最高吸光度值。
The objective of this investigation is to synthesize and investigate zero-valent iron (ZVI) nanoparticles (NPs) for bioremediation applications. The ZVI-NPs were fabricated by chemical reduction using a ferrous salt solution with poly(N-vinylpyrrolidone) (PVP), used as a stabilizer. The synthesis was conducted with and without ultrasonic treatment. The ZVI NPs were fabricated and characterized using scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray powder diffraction (XRD) analysis, and Fourier Transform Infrared Spectroscopy (FTIR). Experimental observations demonstrate that depending on synthesis conditions and coordination of stabilizers, NPs with different morphologies are formed. Colloidal solutions of the synthesized NPs were used in antimicrobial activity tests and biofilm formation assays for nine different control microorganisms: Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 15692), Enterococcus faecalis (ATCC 29122), Klebsiella pneumoniae (laboratory isolates), Proteus vulgaris (laboratory isolates), Staphylococcus aureus (ATCC 29213), Bacillus cereus (DSMZ 4312), Bacillus subtilis (ATCC 6633), and Candida albicans (ATCC 10231). All control strains did not show antibacterial effect against PVP-stabilized ZVI NPs synthesized without ultrasonic treatment. However, biofilm results show that the highest absorbance values of the micro-organisms were tested in control wells.
