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Turbulent motions enhance the diffusion of large-scale flows and temperature gradients. Such diffusion is often parameterized by coefficients of turbulent viscosity ($ν_{\rm t}$) and turbulent thermal diffusivity ($χ_{\rm t}$) that are analogous to their microscopic counterparts. We compute the turbulent diffusion coefficients by imposing large-scale velocity and temperature gradients on a turbulent flow and measuring the response of the system. We also confirm our results using experiments where the imposed gradients are allowed to decay. To achieve this, we use weakly compressible three-dimensional hydrodynamic simulations of isotropically forced homogeneous turbulence. We find that the turbulent viscosity and thermal diffusion, as well as their ratio the turbulent Prandtl number, ${\rm Pr}_{\rm t} = ν_{\rm t}/χ_{\rm t}$, approach asymptotic values at sufficiently high Reynolds and Peclét numbers. We also do not find a significant dependence of ${\rm Pr}_{\rm t}$ on the microscopic Prandtl number ${\rm Pr} = ν/χ$. These findings are in stark contrast to results from the $k-ε$ model which suggests that ${\rm Pr}_{\rm t}$ increases monotonically with decreasing ${\rm Pr}$. The current results are relevant for the ongoing debate of, for example, the nature of the turbulent flows in the very low ${\rm Pr}$ regimes of stellar convection zones.