论文标题
使用咖啡阿拉比卡叶提取物的环保银纳米颗粒的绿色合成，并开发具有成本效益的半胱氨酸的生物传感器
Green synthesis of eco-friendly silver nanoparticles using Coffee arabica leaf extract and development of a cost-effective biosensor for cysteine
论文作者
论文摘要
与涉及其他涉及有毒化学物质的风险干扰的方法相比，研究人员的渴望是通过绿色路线制造环境友好的银纳米颗粒（AGNP）作为通过绿色路线进行生物传感器的渴望，对造成污染的影响较小，对造成污染的影响较小。在这里，首次将咖啡阿拉比卡叶提取物（CAE）以一种简单且环保的方式生产高度稳定的AGNP。在十分钟之内，我们可以通过与CAE添加银硝酸盐（AGNO3）溶液来视觉上确认将Ag+从黄色变为深棕色的AG0。使用Ultraviolet-Visible（UV-VIS）光谱研究研究了CAE还原AgNP（CAE-AGNP）的结构，光学，稳定性，形态和元素特征，傅立叶在ATR模式（FTIR-ATR），Raman Exploscopopicy，Zeta电位分析层和传输层（ZETA）中转化红外光谱镜检查。制备的CAE-AGNP显示出具有高灵敏度的氨基酸L-半胱氨酸（CYS）的良好感应性能，检测限（LOD）为0.1 nm，选择性和良好的稳定性。因此，提出的新方法在医学领域的其他纳米工程绿色AGNP中得到了高度促进，作为有价值的生物传感器。
The thirst for fabricating environment friendly silver nanoparticles (AgNPs) as biosensors via the green route is highly demanded among researchers due to their free of cost and less impact on creating pollution compared to other methods involving risk interference from toxic chemicals as reducing, stabilizing and capping agents. Here, for the first time, Coffee arabica leaf extracts (CAE) were used for the production of highly stable AgNPs in an easy and eco-friendly manner. Within ten minutes, we can visually confirm the reduction of Ag+ to Ag0 from a change of yellow color to dark brown by the addition of silver nitrate (AgNO3) solution with CAE. The structural, optical, stability, morphological and elemental characteristics of CAE-reduced AgNPs (CAE-AgNPs) were investigated using ultraviolet-visible (UV-Vis) spectroscopy, Fourier transforms infrared spectroscopy in ATR mode (FTIR-ATR), Raman spectroscopy, Zeta potential analyzer and transmission electron microscopy (TEM). The prepared CAE-AgNPs show good sensing performance for the amino acid L-cysteine (Cys) with high sensitivity, a limit of detection (LOD) of 0.1 nM, selectivity and good stability. Hence, the proposed novel method is highly promoted among other nanoengineered green AgNPs in the medical field as a valuable biosensor.
