论文标题
希尔伯特阶段显微镜基于Linnik配置中的伪热照明
Hilbert phase microscopy based on pseudo thermal illumination in Linnik configuration
论文作者
论文摘要
定量相显微镜（QPM）通常基于记录对象参考干扰模式及其进一步的相位解调。我们提出了伪希尔伯特阶段显微镜（phpm），在其中结合了伪热光源照明和希尔伯特螺旋变换相位解调，以实现混合硬件软件驱动的噪声稳健性，并增加了单光相干QPM的分辨率。这些有利的特征源于物理改变激光空间相干性和数值恢复频谱重叠的对象空间频率。与激光照明和通过时间相变和傅立叶变换技术相比，PHPM的能力分析了校准的相靶标和活体系细胞。进行的研究验证了phpm对单发成像，噪声最小化和相位细节保存的独特能力。
Quantitative phase microscopy (QPM) is often based on recording an object-reference interference pattern and its further phase demodulation. We propose Pseudo Hilbert Phase Microscopy (PHPM) where we combine pseudo thermal light source illumination and Hilbert spiral transform phase demodulation to achieve hybrid hardware-software-driven noise robustness and increase in resolution of single-shot coherent QPM. Those advantageous features stem from physically altering the laser spatial coherence and numerically restoring spectrally overlapped object spatial frequencies. Capabilities of the PHPM are demonstrated analyzing calibrated phase targets and live HeLa cells in comparison with laser illumination and phase demodulation via temporal phase shifting and Fourier transform techniques. Performed studies verified unique ability of the PHPM to couple single-shot imaging, noise minimization, and preservation of phase details.
