论文标题
石墨烯诱导的能量转移用于亚纳米分辨率下定量膜生物物理学
Graphene-Induced Energy Transfer for Quantitative Membrane Biophysics at Sub-Nanometer Resolution
论文作者
论文摘要
石墨烯诱导的能量转移（GIET）是一种最近开发的荧光 - 光谱技术，可实现沿显微镜光学轴的荧光团的亚纳米光学定位。 Giet基于近场的能量转移从光学激发的荧光分子到单片石墨烯。它已成功地用于估计单脂质双层的叶片间距离，并研究了生活线粒体的膜组织。在这项研究中，我们使用GIET测量胆固醇诱导的纳米级膜厚度的细微变化。我们量化了受支持的脂质双层（SLB）的膜厚度变化，这是脂质组成和胆固醇含量增加的函数。我们的发现表明，Giet是研究生物膜中纳米结构变化的极其敏感的工具。
Graphene-induced energy transfer (GIET) is a recently developed fluorescence-spectroscopic technique that achieves sub-nanometric optical localization of fluorophores along the optical axis of a microscope. GIET is based on the near-field energy transfer from an optically excited fluorescent molecule to a single sheet of graphene. It has been successfully used for estimating inter-leaflet distances of single lipid bilayers, and for investigating the membrane organization of living mitochondria. In this study, we use GIET to measure the cholesterol-induced subtle changes of membrane thickness at the nanoscale. We quantify membrane thickness variations in supported lipid bilayers (SLBs) as a function of lipid composition and increasing cholesterol content. Our findings demonstrate that GIET is an extremely sensitive tool for investigating nanometric structural changes in bio-membranes.
